The Monkey Complement Factor B (CFB) ELISA Kit is a specialized assay designed for the precise quantification of Complement Factor B levels in monkey samples. Complement Factor B is a crucial component of the complement system, playing a key role in the innate immune response and the activation of the alternative complement pathway in primates. This ELISA kit enables researchers to accurately measure CFB concentrations in monkey biological specimens, offering valuable insights into immune system function, complement activation, and inflammatory responses in primate models. By quantifying CFB levels, researchers can investigate immune-related disorders, study complement-mediated diseases, and explore the role of the complement system in immune defense mechanisms in monkeys.
With exceptional sensitivity and specificity, the Monkey CFB ELISA Kit ensures reliable and reproducible results, facilitating in-depth investigations into immune responses, complement pathways, and immune-related conditions in monkey populations. Manufactured under stringent quality control measures, this kit delivers robust performance and user-friendly operation, making it an ideal choice for immunology research and immune function studies in monkeys.
Product Name:
Monkey CFB (Complement Factor B) ELISA Kit
SKU:
AEES00683
Target:
Monkey CFB (Complement Factor B)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
1.88 ng/mL
Detection range:
3.13-200 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey CFB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey CFB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey CFB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey CFB. You can calculate the concentration of Monkey CFB in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
96-112
89-104
Average (%)
97
103
95
1:4
Range (%)
85-100
99-115
86-101
Average (%)
92
105
92
1:8
Range (%)
87-103
95-109
86-98
Average (%)
94
102
91
1:16
Range (%)
91-105
93-110
87-102
Average (%)
99
100
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
97
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.33
26.59
82.88
10.58
24.33
85.57
Standard deviation
0.64
1.29
3.67
0.73
1.4
3.87
C V (%)
6.2
4.85
4.43
6.9
5.75
4.52
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey CFB concentrations in .
Specificity:
This kit recognizes Monkey CFB in samples. No significant cross-reactivity or interference between Monkey CFB and analogues was observed.
