The Monkey C3a (Complement Component 3a) ELISA Kit is specifically designed for the accurate measurement of C3a levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and reliable results, making it an ideal tool for various research applications.Complement Component 3a is a critical component of the complement system, playing a key role in inflammation and immune response regulation. Dysregulation of C3a has been implicated in various diseases, including autoimmune disorders, infectious diseases, and inflammatory conditions.
Therefore, measuring C3a levels can provide valuable insights into the pathogenesis of these diseases and aid in the development of targeted therapies.Whether studying immune responses, inflammatory pathways, or disease mechanisms, the Monkey C3a ELISA Kit offers researchers a reliable and efficient solution for quantifying C3a levels in monkey samples. With its ease of use and high performance, this kit is a valuable asset for advancing scientific understanding and potential therapeutic strategies.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey C3a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey C3a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey C3a. You can calculate the concentration of Monkey C3a in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
94-110
87-98
Average (%)
92
100
92
1:4
Range (%)
84-98
93-108
83-94
Average (%)
91
101
88
1:8
Range (%)
87-97
93-106
87-98
Average (%)
92
99
92
1:16
Range (%)
83-94
90-106
83-95
Average (%)
89
97
89
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-101
95
EDTA plasma (n=5)
94-107
101
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.48
1.46
3.47
0.51
1.58
3.28
Standard deviation
0.03
0.08
0.18
0.03
0.08
0.16
C V (%)
6.25
5.48
5.19
5.88
5.06
4.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey C3a concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey C3a in samples. No significant cross-reactivity or interference between Monkey C3a and analogues was observed.
