The Monkey C3 (Complement Component 3) ELISA Kit is a cutting-edge tool tailored for the precise quantitative assessment of C3 levels in diverse biological samples derived from monkeys. Complement Component 3 is a key player in the intricate immune system, instrumental in opsonization, inflammation, and immune complex clearance. This specialized ELISA kit empowers researchers to delve into the immune response dynamics and decode the role of C3 in immune-related pathologies and disorders commonly observed in primate models. Accurate quantification of C3 is indispensable for unraveling its immunological functions and understanding its implications in immune-related diseases and conditions.
Developed with top-notch sensitivity and specificity, the Monkey C3 ELISA Kit ensures dependable and reproducible results, enabling scientists to investigate the role of C3 with confidence. Crafted under strict quality control measures, this kit offers robust performance and user-friendly protocols, making it a premier choice for research studies focused on immunoassays and immunological investigations in primate settings.
Product Name:
Monkey C3 (Complement Component 3) ELISA Kit
SKU:
AEES00271
Target:
Monkey C3 (Complement Component 3)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.47 ng/mL
Detection range:
0.78-50 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey C3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey C3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey C3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey C3. You can calculate the concentration of Monkey C3 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-110
89-99
92-107
Average (%)
103
94
99
1:4
Range (%)
97-115
83-95
94-107
Average (%)
105
89
102
1:8
Range (%)
96-110
84-100
93-108
Average (%)
104
91
101
1:16
Range (%)
97-110
92-105
97-111
Average (%)
103
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
84-95
90
Cell culture media (n=5)
88-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2.6
6.03
24.21
2.54
6.55
25.76
Standard deviation
0.13
0.34
1.01
0.17
0.38
1.07
C V (%)
5.18
5.71
4.16
6.64
5.73
4.14
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey C3 concentrations in .
Specificity:
This kit recognizes Monkey C3 in samples. No significant cross-reactivity or interference between Monkey C3 and analogues was observed.
