The Monkey C1q (Complement 1q) ELISA Kit is a specialized assay developed for the quantitative measurement of C1q levels in diverse biological samples. C1q is a crucial component of the complement system, participating in immune responses, inflammation, and clearance of pathogens and apoptotic cells. This ELISA kit provides researchers with the means to accurately quantify C1q levels, enabling investigations into its role in immune regulation and disease pathology in primates. Accurate assessment of C1q concentration is vital for understanding its involvement in complement-mediated pathways, autoimmune disorders, and infection responses.
The Monkey C1q ELISA Kit ensures high sensitivity and specificity, delivering precise and reproducible results tailored to your research needs. With stringent quality control measures in place, this kit offers robust performance and user-friendly protocols, making it an excellent tool for primate studies focused on the functions and implications of C1q.
Product Name:
Monkey C1q (Complement 1q) ELISA Kit
SKU:
AEES00269
Target:
Monkey C1q (Complement 1q)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey C1q. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey C1q, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey C1q. You can calculate the concentration of Monkey C1q in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-108
90-96
92-106
Average (%)
100
93
100
1:4
Range (%)
87-95
83-95
86-94
Average (%)
92
90
90
1:8
Range (%)
83-93
94-103
85-95
Average (%)
90
98
91
1:16
Range (%)
98-107
85-101
86-96
Average (%)
102
93
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
88-102
96
EDTA plasma (n=5)
99-110
102
Cell culture media (n=5)
98-108
102
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.99
2.28
8.3
0.8
2.23
8.83
Standard deviation
0.05
0.11
0.4
0.05
0.11
0.39
C V (%)
5.05
4.82
4.82
6.25
4.93
4.42
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey C1q concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey C1q in samples. No significant cross-reactivity or interference between Monkey C1q and analogues was observed.
