The Monkey BALP (Bone Alkaline Phosphatase) ELISA Kit is specifically designed for the accurate measurement of BALP levels in monkey serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research purposes.Bone Alkaline Phosphatase is an important enzyme involved in bone mineralization and is commonly used as a marker for bone formation and turnover.
Monitoring BALP levels can provide valuable insights into bone health and various bone-related diseases, making this kit an essential tool for research in the fields of osteoporosis, skeletal development, and bone metabolism.With its reliable performance and ease of use, the Monkey BALP ELISA Kit is an indispensable resource for researchers studying bone biology and seeking to understand the mechanisms underlying bone diseases.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey ALPL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey ALPL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey ALPL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey ALPL. You can calculate the concentration of Monkey ALPL in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-103
87-101
90-101
Average (%)
96
92
96
1:4
Range (%)
89-105
80-93
89-99
Average (%)
96
85
94
1:8
Range (%)
87-98
80-92
83-99
Average (%)
93
86
90
1:16
Range (%)
90-102
88-101
87-101
Average (%)
96
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-106
96
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
88-100
93
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.46
1.57
4.73
0.47
1.54
4.76
Standard deviation
0.03
0.09
0.24
0.03
0.09
0.16
C V (%)
6.52
5.73
5.07
6.38
5.84
3.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey ALPL concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey ALPL in samples. No significant cross-reactivity or interference between Monkey ALPL and analogues was observed.
