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Monkey Alpha-Fetoprotein (aFP) ELISA Kit

SKU:
MKDL00001
Product Type:
ELISA Kit
Sensitivity:
1.2ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
A-FP,FETA,HPAFP,Alpha-Fetoglobulin,Alpha-1-fetoprotein
Reactivity:
Monkey
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€749
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Description

system_update_altDatasheet

Monkey Alpha-Fetoprotein (aFP) ELISA Kit

The Monkey Alpha-fetoprotein (AFP) ELISA Kit offered by Assay Genie is a powerful tool for accurately measuring AFP levels in monkey serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, researchers can trust in the reliability and reproducibility of their results for a variety of research applications.Alpha-fetoprotein is a key protein involved in fetal development and also serves as a biomarker for various liver diseases and certain types of cancer in monkeys.

By utilizing this ELISA kit, researchers can gain valuable insights into the role of AFP in these conditions, paving the way for potential therapeutic advancements. Trust in Assay Genie's Monkey AFP ELISA Kit for precise and informative results in your research endeavors.

Product Name:Monkey Alpha-Fetoprotein (aFP) ELISA Kit
Product Code:MKDL00001
Size:96 Assays
Target:aFP
Synonyms:A-FP, FETA, HPAFP, Alpha-Fetoglobulin, Alpha-1-fetoprotein
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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