The Monkey Angiotensinogen (AGT) ELISA Kit is a specialized assay designed for the quantitative measurement of Angiotensinogen levels in monkey samples. Angiotensinogen is a vital protein precursor involved in the renin-angiotensin system, playing a pivotal role in regulating blood pressure, fluid balance, and electrolyte homeostasis in primates. This ELISA kit offers researchers a reliable tool to accurately quantify Angiotensinogen concentrations in monkey biological specimens, providing valuable insights into the intricate mechanisms of cardiovascular regulation, renal function, and blood pressure control in non-human primates. By assessing AGT levels, researchers can explore the impact of this essential protein on vascular health, hypertension, and kidney physiology in monkey models.
With exceptional specificity and sensitivity, the Monkey AGT ELISA Kit ensures precise and reproducible results, facilitating investigations into the role of Angiotensinogen in the pathophysiology of cardiovascular diseases, renal disorders, and hypertension in primate research. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly protocols, making it an excellent choice for cardiovascular and hypertension studies in monkey models.
Product Name:
Monkey AGT (Angiotensinogen) ELISA Kit
SKU:
AEES00686
Target:
Monkey AGT (Angiotensinogen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey AGT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey AGT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey AGT. You can calculate the concentration of Monkey AGT in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-103
88-98
87-99
Average (%)
95
93
94
1:4
Range (%)
85-96
82-95
85-95
Average (%)
90
89
90
1:8
Range (%)
89-103
85-97
89-101
Average (%)
96
92
94
1:16
Range (%)
86-98
85-99
83-98
Average (%)
92
91
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-96
91
EDTA plasma (n=5)
90-100
95
Cell culture media (n=5)
93-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.97
14.22
34.14
4.59
15.5
36.41
Standard deviation
0.33
0.77
1.45
0.23
0.7
1.11
C V (%)
6.64
5.41
4.25
5.01
4.52
3.05
Sample type &Sample volume:
; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey AGT concentrations in .
Specificity:
This kit recognizes Monkey AGT in samples. No significant cross-reactivity or interference between Monkey AGT and analogues was observed.
