Monkey A beta1-40 (Amyloid Beta Peptide 1-40) ELISA Kit (MKES00010)
The Monkey Aβ (Beta1-40) Amyloid Beta Peptide 1-40 ELISA Kit is a reliable tool for accurate quantification of Aβ (Beta1-40) levels in monkey serum, plasma, and cell culture supernatants. This kit is specifically designed for high sensitivity and specificity, guaranteeing precise and consistent results for various research applications.Amyloid Beta (Aβ) peptides are important in the pathogenesis of Alzheimer's disease, and accurately measuring their levels is critical for studying disease progression and potential therapeutic interventions.
This ELISA kit enables researchers to efficiently analyze Aβ (Beta1-40) levels in monkey samples, providing valuable insights into disease mechanisms and treatment strategies.Overall, the Monkey Aβ (Beta1-40) Amyloid Beta Peptide 1-40 ELISA Kit is a valuable tool for researchers investigating the role of Aβ peptides in neurodegenerative disorders and advancing the development of novel therapeutic approaches.
Product Name:
Monkey A beta1-40 (Amyloid Beta Peptide 1-40) ELISA Kit (MKES00010)
SKU:
MKES00010
Target:
Monkey A beta1-40 (Amyloid Beta Peptide 1-40)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.09 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey Aβ1-40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey Aβ1-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey Aβ1-40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey Aβ1-40. You can calculate the concentration of Monkey Aβ1-40 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-106
87-99
94-110
Average (%)
97
94
101
1:4
Range (%)
86-99
84-98
96-107
Average (%)
93
90
101
1:8
Range (%)
88-101
89-103
94-110
Average (%)
94
95
101
1:16
Range (%)
92-103
83-96
92-104
Average (%)
98
90
97
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-103
97
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
90-104
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.53
0.84
4.62
0.56
0.88
5.03
Standard deviation
0.03
0.03
0.18
0.03
0.05
0.15
C V (%)
5.66
3.57
3.9
5.36
5.68
3.05
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Monkey Aβ1-40 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Monkey Aβ1-40 in samples. No significant cross-reactivity or interference between Monkey Aβ1-40 and analogues was observed.
