The MMP24 Polyclonal Antibody (PAC018240) is a valuable tool for researchers studying matrix metalloproteinase 24 (MMP24), a key enzyme involved in matrix remodeling and tissue repair processes. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the MMP24 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for investigations in the fields of biology, medicine, and pharmaceutical research.
MMP24, also known as MT5-MMP, is a member of the matrix metalloproteinase family that plays a crucial role in extracellular matrix degradation and cell signaling pathways. Its diverse functions in tissue remodeling and cell migration make it a promising target for studies related to cancer metastasis, wound healing, and neuroinflammatory disorders. By elucidating the mechanisms underlying MMP24 activity, researchers can gain valuable insights into disease progression and potentially identify novel therapeutic strategies for addressing these conditions.
Antibody Name:
MMP24 Antibody (PACO18240)
Antibody SKU:
PACO18240
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Synthetic peptide of human MMP24
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 6%SDS-PAGE,Lysate: 40 μg,Lane 1-2: 293T cells, HepG2 cells,Primary antibody: PACO18240(MMP24 Antibody) at dilution 1/200 dilution,Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution,Exposure time: 40 seconds.
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
Synonyms:
matrix metallopeptidase 24 (membrane-inserted)
UniProt Protein Function:
MMP24: Activates progelatinase A. May also be a proteoglycanase involved in degradation of proteoglycans, such as dermatan sulfate and chondroitin sulfate proteoglycans. Cleaves partially fibronectin, but not collagen type I, nor laminin. Belongs to the peptidase M10A family.
UniProt Protein Details:
Protein type:Membrane protein, integral; EC 3.4.24.-; Activator; Protease
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: proteinaceous extracellular matrix; integral to plasma membrane; trans-Golgi network membrane
Molecular Function:zinc ion binding; cadherin binding; metalloendopeptidase activity; enzyme activator activity; calcium ion binding
Biological Process: positive regulation of catalytic activity; glial cell differentiation; proteolysis; detection of temperature stimulus involved in sensory perception of pain
NCBI Summary:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP25 but has been renamed MMP24. [provided by RefSeq, Jul 2008]
