The MMP2 Monoclonal Antibody (PAC064303) is a valuable tool for researchers studying matrix metalloproteinase 2 (MMP2), an enzyme involved in extracellular matrix remodeling and implicated in various pathological conditions including cancer, inflammation, and tissue repair. This antibody, produced in mice, is highly specific to human MMP2 and has been validated for use in immunohistochemistry and Western blot applications.MMP2 is a key player in cancer progression, as it promotes tumor invasion and metastasis by breaking down extracellular matrix components. Additionally, MMP2 is involved in physiological processes such as wound healing and angiogenesis.
Understanding the regulation and function of MMP2 is critical for developing targeted therapies for cancer and other diseases characterized by abnormal extracellular matrix remodeling.The MMP2 Monoclonal Antibody enables researchers to detect and quantify MMP2 protein expression in various sample types, providing valuable insights into the role of MMP2 in different biological processes. Its high specificity and reliability make it a powerful tool for studying MMP2 biology and developing novel therapeutic strategies targeting this enzyme.
Antibody Name:
Mmp2 Antibody (PACO64303)
Antibody SKU:
PACO64303
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:1000-1:5000
Species Reactivity:
Mouse, Human
Immunogen:
Recombinant Mouse 72 kDa type IV collagenase protein (412-662AA)
Western Blot. Positive WB detected in: Hela whole cell lysate. All lanes: Mmp2 antibody at 1:2000. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 75, 66 kDa. Observed band size: 75 kDa.
Background:
Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly- |-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3β in vitro. Involved in the formation of the fibrovascular tissues (By similarity).By similarityPEX, the C-terminal non-catalytic fragment of MMP2, posseses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. Ligand for integrin alpha-v/beta-3 on the surface of blood vessels (By similarity).By similarityIsoform 2: Mediates the proteolysis of CHUK/IKKA and initiates a primary innate immune response by inducing mitochondrial-nuclear stress signaling with activation of the pro-inflammatory NF-kappaB, NFAT and IRF transcriptional pathways.
Synonyms:
72 kDa type IV collagenase (EC 3.4.24.24) (72 kDa gelatinase) (Gelatinase A) (Matrix metalloproteinase-2) (MMP-2) [Cleaved into: PEX], Mmp2
UniProt Protein Function:
MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly-|-Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) with the integrin alpha-V/beta-3; the interaction promotes vascular invasion in angiogenic vessels and melamoma cells. Interacts (via the C-terminal PEX domain) with TIMP2 (via the C-terminal); the interaction inhibits the degradation activity. Interacts with GSK3B. Aspirin appears to inhibit expression. Produced by normal skin fibroblasts. PEX is expressed in a number of tumors including gliomas, breast and prostate. Inhibited by histatin-3 1/24 (histatin-5). Belongs to the peptidase M10A family.
UniProt Protein Details:
Protein type:Protease; Secreted; Apoptosis; Cell development/differentiation; EC 3.4.24.24; Secreted, signal peptide; Motility/polarity/chemotaxis
72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2
