The MMP14 Polyclonal Antibody (CAB2549) is a crucial tool for researchers studying Matrix Metalloproteinase 14 (MMP14), an enzyme involved in extracellular matrix degradation and tissue remodeling. Raised in rabbits, this antibody is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the MMP14 protein, this antibody enables researchers to detect and analyze MMP14 expression in various cell types, aiding studies in cancer progression, metastasis, and tissue repair mechanisms.MMP14, also known as membrane-type matrix metalloproteinase 1 (MT1-MMP), plays a key role in regulating cell invasion, migration, and angiogenesis.
Dysregulation of MMP14 has been implicated in various pathological conditions such as cancer, arthritis, and cardiovascular diseases. This antibody's ability to target MMP14 provides researchers with a valuable tool for understanding the molecular mechanisms underlying these diseases and developing targeted therapies to inhibit MMP14 activity. Unlock the potential of MMP14 research with the MMP14 Polyclonal Antibody (CAB2549).
Product Name:
MMP14/MT1-MMP Rabbit Polyclonal Antibody
SKU:
CAB2549
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 75-174 of human MMP14/MMP14/MT1-MMP (NP_004986.1).
Cytoplasm, Melanosome, Membrane, Single-pass type I membrane protein.
Calculated MW:
66kDa
Observed MW:
60kDa
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.
Purification Method:
Affinity purification
Gene ID:
4323
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat spleen using MMP14/MT1-MMP Rabbit pAb(CAB2549) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
