The MMACHC Polyclonal Antibody (CAB10355) is a valuable tool for researchers studying MMACHC, a protein involved in the cellular metabolism of vitamin B12. This antibody, generated in rabbits, exhibits high reactivity to human samples and has been validated for use in Western blot applications. By specifically binding to the MMACHC protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component for investigations in biochemistry and molecular biology.MMACHC plays a crucial role in the intracellular processing of vitamin B12, a vital nutrient involved in various physiological processes, including DNA synthesis and neurological function. Dysregulation of MMACHC function has been linked to the development of disorders related to vitamin B12 deficiency, such as megaloblastic anemia and neurological complications.
Therefore, understanding the mechanisms underlying MMACHC activity is essential for advancing research aimed at developing treatments for these conditions.Overall, the MMACHC Polyclonal Antibody (CAB10355) is a reliable reagent that offers researchers a means to explore the functions and regulation of MMACHC in both health and disease. Its versatility and specificity make it an invaluable asset for studies focused on vitamin B12 metabolism, paving the way for advancements in the field of metabolism and nutrient processing.
Product Name:
MMACHC Rabbit Polyclonal Antibody
SKU:
CAB10355
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 63-282 of human MMACHC (NP_056321.2).
The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC.
Purification Method:
Affinity purification
Gene ID:
25974
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MMACHC antibody (CAB10355) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 20s.
