The MLKL Polyclonal Antibody (CAB5579) is a valuable tool for researchers studying mixed lineage kinase domain-like protein (MLKL), a critical player in the necroptosis cell death pathway. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications.MLKL is a key mediator of necroptosis, a form of programmed cell death that has been implicated in a variety of pathological conditions, including inflammatory diseases, neurodegenerative disorders, and cancer. By targeting MLKL with this antibody, researchers can effectively detect and analyze the protein in various cell types, providing insight into the mechanisms underlying necroptosis and its role in disease progression.
The MLKL Polyclonal Antibody is a valuable tool for investigators working in the fields of cell biology, immunology, and oncology. Its ability to specifically bind to MLKL makes it an essential component for studies aimed at understanding the molecular pathways involved in necroptosis and developing targeted therapies for diseases associated with dysregulated cell death processes.
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
Email for sequence
Gene ID:
74568
Uniprot:
Q9D2Y4
Cellular Location:
Cell membrane, Cytoplasm
Calculated MW:
53kDa/54kDa
Observed MW:
54kDa
Synonyms:
MLKL, hMLKL
Background:
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:
MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; TKL group; TKL-Unique familyCellular Component: membrane; cytoplasm; plasma membraneMolecular Function: transferase activity, transferring phosphorus-containing groups; protein complex binding; nucleotide binding; protein kinase binding; ATP binding; protein kinase activityBiological Process: programmed cell death; protein amino acid phosphorylation
UniProt Protein Details:
NCBI Summary:
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015]
Western blot analysis of extracts of various cell lines, using MLKL antibody (CAB5579) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
