The MLKL Polyclonal Antibody (CAB13452) is a valuable tool for researchers studying the MLKL protein, a key player in the necroptosis cell death pathway. This antibody, produced in rabbits, shows high reactivity with human samples, making it ideal for Western blot applications. By binding to the MLKL protein, this antibody enables precise detection and analysis in a variety of cell types, facilitating research in fields such as cell biology, apoptosis, and cancer.MLKL, or mixed lineage kinase domain-like protein, is essential for triggering necroptosis, a form of programmed cell death that plays a role in immune defense and inflammation.
Dysregulation of the necroptosis pathway has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune conditions. Therefore, investigating the function of MLKL and its interactions is crucial for understanding disease mechanisms and developing targeted therapies.In summary, the MLKL Polyclonal Antibody (CAB13452) is a reliable tool for researchers interested in studying the role of MLKL in necroptosis and its implications for human health. Its specificity and sensitivity make it a valuable asset for projects aiming to uncover novel therapeutic targets and mechanisms of cell death.
Antibody Name:
Anti-MLKL Antibody
Antibody SKU:
CAB13452
Antibody Size:
20uL, 50uL, 100uL
Application:
WB IHC
Reactivity:
Human, Mouse, Rat
Host Species:
Rabbit
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of mouse MLKL (NP_001297542.1).
Application:
WB IHC
Recommended Dilution:
WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:
Human, Mouse, Rat
Positive Samples:
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 400 to the C-terminus of mouse MLKL (NP_001297542.1).
Purification Method:
Affinity purification
Storage Buffer:
Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:
IgG
Sequence:
EGCD SKKI RELV AEDK KQEP VGQD CPEL LREI INEC RAHE PSQR PSVD GRSL SGRE RILE RLSA VEES TDKK V
Gene ID:
74568
Uniprot:
Cellular Location:
Cell membrane, Cytoplasm
Calculated MW:
53kDa/54kDa
Observed MW:
Refer to figures
Synonyms:
MLKL, hMLKL
Background:
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Immunohistochemistry of paraffin-embedded human tonsil using MLKL antibody (CAB13452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using MLKL antibody (CAB13452) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterine cancer using MLKL antibody (CAB13452) at dilution of 1:100 (40x lens).
