The MLKL Polyclonal Antibody (CAB13451) is a valuable tool for researchers studying the programmed cell death pathway and its implications in various diseases such as cancer and autoimmune disorders. This antibody, generated in rabbits, specifically targets mixed lineage kinase domain-like protein (MLKL) and is highly reactive with human samples.MLKL is a key player in necroptosis, a form of programmed cell death that has been linked to inflammation, immune response, and tissue damage. By utilizing this antibody in Western blot applications, researchers can accurately detect and analyze MLKL in different cell types, providing insights into the mechanisms of cell death and potential therapeutic targets.
The importance of understanding MLKL and its role in necroptosis cannot be understated, especially in the context of developing targeted therapies for diseases where necroptosis plays a significant role. By using the MLKL Polyclonal Antibody, researchers can advance their studies in cell death pathways and contribute to the development of novel treatment strategies.
Product Name:
[KO Validated] MLKL Rabbit Polyclonal Antibody
SKU:
CAB13451
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-62 of mouse MLKL (Q9D2Y4).
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
74568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MLKL Rabbit pAb (CAB13451) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
