The MLKL Polyclonal Antibody (CAB13450) is a crucial tool for researchers studying mixed lineage kinase domain-like protein (MLKL), a key player in the complex process of necroptosis. Necroptosis is a form of programmed cell death that differs from apoptosis and has been implicated in various diseases, including inflammatory disorders and cancer.Raised in rabbits, this antibody has been rigorously validated for use in Western blot applications, allowing for the detection and analysis of MLKL protein levels in human samples.
By binding specifically to MLKL, researchers can gain insights into the mechanisms underlying necroptosis and its role in disease pathogenesis.Given the emerging importance of necroptosis in various pathological conditions, the MLKL Polyclonal Antibody is a valuable tool for researchers interested in understanding and targeting this cell death pathway for therapeutic purposes. Its high reactivity and specificity make it an ideal choice for immunology and cancer research studies focused on necroptosis.
Product Name:
MLKL Rabbit Polyclonal Antibody
SKU:
CAB13450
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-62 of mouse MLKL (Q9D2Y4).
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to lack protein kinase activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (Rip3), which is a key signaling molecule in necroptosis pathway. Knockout of this gene in mice showed that it is essential for necroptosis. Alternatively spliced transcript variants have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
74568
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HT-29 cells, using MLKL Rabbit pAb (CAB13450) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
