The MLC1 Polyclonal Antibody (CAB12840) is a highly specific and sensitive tool for investigating the protein MLC1, which is associated with megalencephalic leukoencephalopathy with subcortical cysts (MLC), a rare genetic disorder affecting the brain. This antibody, raised in rabbits, is validated for use in various applications, including Western blot and immunofluorescence, enabling researchers to study the expression and localization of MLC1 in different cell types and tissues.MLC1 is a transmembrane protein primarily expressed in astrocytes and oligodendrocytes in the brain, where it plays a crucial role in maintaining ion and water homeostasis in the central nervous system.
Mutations in the MLC1 gene lead to dysregulation of these processes, resulting in the characteristic white matter abnormalities seen in MLC patients.By using the MLC1 Polyclonal Antibody, researchers can further explore the function and regulation of MLC1 in normal brain development and function, as well as in the pathogenesis of MLC. This antibody is a valuable tool for studying the molecular mechanisms underlying MLC and developing potential therapeutic strategies for this devastating neurological disorder.
Product Name:
MLC1 Rabbit Polyclonal Antibody
SKU:
CAB12840
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human MLC1 (NP_055981.1).
The function of this gene product is unknown; however, homology to other proteins suggests that it may be an integral membrane transporter. Mutations in this gene have been associated with megalencephalic leukoencephalopathy with subcortical cysts, an autosomal recessive neurological disorder. Alternatively spliced transcript variants encoding different isoforms have been identified.
Purification Method:
Affinity purification
Gene ID:
23209
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from SH-SY5Y cells, using MLC1 Rabbit pAb (CAB12840) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
