The MKX Polyclonal Antibody (PAC058284) is a specialized research tool for studying MKX, a key transcription factor involved in musculoskeletal development and homeostasis. This antibody, developed using innovative techniques, exhibits strong reactivity with human samples and has been rigorously validated for Western blot applications.MKX, also known as Mohawk homeobox, is essential for proper tendon and ligament development in the musculoskeletal system. Its role in regulating gene expression and tissue differentiation makes it a valuable target for investigating musculoskeletal disorders, such as tendon injuries and osteoarthritis.
Researchers can utilize the MKX Polyclonal Antibody to detect and analyze MKX protein levels in various cell types, providing crucial insights into its function and mechanisms of action. This antibody is a valuable tool for advancing research in musculoskeletal biology and developing novel therapeutic strategies for musculoskeletal conditions.
Western Blot. Positive WB detected in: SH-SY5Y whole cell lysate. All lanes: MKX antibody at 6µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40 kDa. Observed band size: 40 kDa.
Immunofluorescence staining of Hela cells with PACO58284 at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC image of PACO58284 diluted at 1:400 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
May act as a morphogenetic regulator of cell adhesion.
Synonyms:
Homeobox protein Mohawk, MKX, C10orf48 IRXL1
UniProt Protein Function:
MKX: May act as a morphogenetic regulator of cell adhesion. Belongs to the TALE/IRO homeobox family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 10p12.1Biological Process: muscle development
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is an IRX family-related homeobox protein that may play a role in cell adhesion. Studies in mice suggest that this protein may be a regulator of tendon development. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jun 2011]
