The MKL1 Polyclonal Antibody (CAB12598) is a valuable tool for researchers studying MKL1, a transcriptional coactivator involved in cellular processes such as gene expression, cell migration, and cytoskeletal organization. Raised in rabbits, this antibody has high reactivity with human samples and has been validated for use in Western blot applications.MKL1, also known as Megakaryoblastic Leukemia 1, plays a crucial role in regulating gene expression in response to various signaling pathways, including those involved in cell growth and differentiation. Dysregulation of MKL1 has been implicated in diseases such as cancer and cardiovascular disorders, making it a promising target for therapeutic interventions.
By utilizing the MKL1 Polyclonal Antibody, researchers can effectively detect and analyze MKL1 protein levels in different cell types, providing insights into its function and potential implications in disease pathology. This antibody is an essential tool for studies in molecular biology, cancer research, and developmental biology, offering valuable information that can lead to the development of novel therapeutic strategies targeting MKL1.
Product Name:
MKL1 Rabbit Polyclonal Antibody
SKU:
CAB12598
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
The protein encoded by this gene interacts with the transcription factor myocardin, a key regulator of smooth muscle cell differentiation. The encoded protein is predominantly nuclear and may help transduce signals from the cytoskeleton to the nucleus. This gene is involved in a specific translocation event that creates a fusion of this gene and the RNA-binding motif protein-15 gene. This translocation has been associated with acute megakaryocytic leukemia. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
57591
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MKL1 Rabbit pAb (CAB12598) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
