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Anti-Ki67 Antibody (CAB2094)

SKU:
CAB2094
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB2094
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB2094
  • Cell Cycle Antibodies 1 Anti-Ki67 Antibody CAB2094
€129 - €359
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Description

Anti-Ki67 Antibody

Ki67 protein is expressed by dividing cells but is rarely found in resting cells which makes it a reliable marker for cell proliferation. The presence of Ki67 can be used to infer how well cancer therapy has worked on cancer cells and the rate of tumor growth. Proliferating cancer cells are sensitive to chemotherapy, while non proliferating cells are generally not affected by such treatments. Ki67 is often used as a marker in assays to test various chemotherapies on tumors that display high proliferation (such as neuroblastomas).

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-Ki67 Antibody
Antibody SKU:CAB2094
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1300 of human Ki67 (NP_001139438.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, U-251MG, Mouse testis
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1200-1300 of human Ki67 (NP_001139438.1).
Purification Method:Affinity purification
Storage Buffer: Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence: QKLD LTEN LTGS KRRL QTPK EKAQ ALED LAGF KELF QTRG HTEE SMTN DKTA KVAC KSSQ PDPD KNPA SSKR RLKT SLGK VGVK EELL AVGK LTQT SGET T
NCBI Gene ID:4288
UniProt Code:P46013
Cellular Location:Chromosome, Nucleus, nucleolus
Calculated MW:319kDa/358kDa
Observed MW:359KDa
Synonyms: MKI67, KIA, MIB-, MIB-1, PPP1R105, marker of proliferation Ki-67, Ki67
Background: This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.
NCBI GenInfo Identifier:118572663
NCBI Accession:P46013.2
Anti-Ki67 Antibody (CAB2094) Western blot analysis of extracts of various cell lines, using Ki67 Rabbit pAb (CAB2094) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-Ki67 Antibody (CAB2094) Immunohistochemistry of paraffin-embedded human lung cancer using Ki67 antibody (CAB2094) at dilution of 1:200 (40x lens).
Anti-Ki67 Antibody (CAB2094) Immunofluorescence analysis of HeLa cells using Ki67 antibody (CAB2094) at dilution of 1:100. Blue: DAPI for nuclear staining.
Novitasari et al.PGV-1 permanently arrests HepG2 cells in M phase and inhibits liver carcinogenesis in DMH-induced ratsJournal of Applied Pharmaceutical Science 2023View Citation

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