Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit
- SKU:
- MAES0224
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Animal Tissue
- Sensitivity:
- 3.0 U/L
- Range:
- 3.0-88.6 U/L
- Assay Time:
- 10 min
Frequently bought together:
Description
Product Name: | Mitochondrial Complex IV (Cytochrome C Oxidase ) Activity Assay Kit |
Product Code: | MAES0224 |
Sample Type: | Animal Tissue |
Assay Time: | 10 min |
Assay Type: | Enzyme Activity |
Detection Instrument: | Microplate reader (540-560 nm, optimum wavelength: 550 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 3.0 U/L |
Detection Range: | 3.0–88.6 U/L |
Inter-assay CV: | 0.095 |
Intra-assay CV: | 0.05 |
Mitochondrial complex IV can catalyze the oxidation of reduced cytochrome C to oxidized cytochrome C, which has an absorption wavelength at 550 nm. Therefore, the activity of mitochondrial complex IV can be quantified by measure the change OD value at 550 nm.
Storage: | -20°C |
Valid Period: | 12 months |
Other Reagents Required: | Anhydrous ethanol, PBS(0.01 M,pH 7.4) |
Other Instruments Required: | Centrifuge |