The MITF Polyclonal Antibody (CAB1255) is a valuable tool for researchers studying the transcription factor MITF (Microphthalmia-associated transcription factor), which plays a key role in the regulation of melanocyte development and pigmentation. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot and immunohistochemistry applications.MITF is essential for melanogenesis, the process by which melanocytes produce melanin, the pigment responsible for skin, hair, and eye color. Dysregulation of MITF has been implicated in various skin disorders, including melanoma, making it a significant target for cancer research.
By binding to MITF, this antibody enables researchers to detect and analyze the protein in a variety of cell types, providing valuable insights into melanocyte biology and melanoma development.In addition to its role in melanogenesis, MITF has also been linked to other cellular processes, such as cell proliferation, survival, and differentiation. Understanding the mechanisms by which MITF regulates these processes is of great interest in the fields of developmental biology, cancer biology, and regenerative medicine. The MITF Polyclonal Antibody is a versatile tool that can aid researchers in unraveling the complex functions of this important transcription factor.
Product Name:
MITF Rabbit Polyclonal Antibody
SKU:
CAB1255
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MITF (NP_937801.1).
The protein encoded by this gene is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. The encoded protein regulates melanocyte development and is responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome.
Purification Method:
Affinity purification
Gene ID:
4286
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MITF antibody (CAB1255) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
