The MINK1 Polyclonal Antibody (PAC021895) is a valuable tool for researchers interested in studying the MINK1 protein, a key regulator of signaling pathways involved in cell growth and survival. This antibody, generated in rabbits, exhibits high specificity for human samples and has been validated for use in Western blotting applications.MINK1 is a serine/threonine protein kinase that plays a critical role in various cellular processes, including cell proliferation, survival, and migration.
Dysregulation of MINK1 signaling has been implicated in diseases such as cancer, making it a promising target for therapeutic intervention.By targeting the MINK1 protein, this antibody enables researchers to detect and analyze MINK1 expression in a variety of cell types, providing valuable insights into the role of MINK1 in cellular pathways and disease processes. Its versatility and specificity make it an ideal tool for studies in cell biology, cancer research, and drug discovery.
Antibody Name:
MINK1 Antibody (PACO21895)
Antibody SKU:
PACO21895
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from internal of human MAP4K6.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from COLO cells, using MAP4K6 antibody.
Background:
Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1. Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration. Beausoleil S.A., Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
MINK: a protein kinase of the STE20 family. Studies of the mouse ortholog indicate increased expression during postnatal mouse cerebral development. Activates JNK and the p38 pathways. Three splicing isoforms have been described.
UniProt Protein Details:
Protein type:EC 2.7.11.1; Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; MSN subfamily
Molecular Function:ATP binding; MAP kinase kinase kinase kinase activity; protein binding; protein kinase activity; protein serine/threonine kinase activity
Biological Process: actin cytoskeleton reorganization; dendrite morphogenesis; JNK cascade; microvillus biogenesis; multicellular organismal development; negative thymic T cell selection; neurite morphogenesis; positive regulation of JNK cascade; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of cell migration; regulation of cell-cell adhesion; regulation of cell-matrix adhesion; regulation of mitotic cell cycle; response to stress; synaptic transmission
NCBI Summary:
This gene encodes a serine/threonine kinase belonging to the germinal center kinase (GCK) family. The protein is structurally similar to the kinases that are related to NIK and may belong to a distinct subfamily of NIK-related kinases within the GCK family. Studies of the mouse homolog indicate an up-regulation of expression in the course of postnatal mouse cerebral development and activation of the cJun N-terminal kinase (JNK) and the p38 pathways. [provided by RefSeq, Mar 2016]
