The MICAL2 Monoclonal Antibody (PACO28238) is a valuable tool for researchers studying MICAL2, a protein involved in cellular processes such as cytoskeletal dynamics and membrane trafficking. This monoclonal antibody, produced using hybridoma technology, is highly specific to human MICAL2 and has been validated for use in immunofluorescence and immunohistochemistry applications.MICAL2, a member of the MICAL family of proteins, plays a role in cell signaling pathways and has been implicated in diseases such as cancer and neurodegenerative disorders.
By targeting MICAL2 with this antibody, researchers can investigate its localization and function within cells, providing insights into its role in disease progression and potential therapeutic strategies.Overall, the PACO28238 monoclonal antibody offers a powerful tool for studying MICAL2 biology and its implications in various disease states, making it an essential component for research in the fields of cell biology and molecular medicine.
Immunohistochemistry of paraffin-embedded human pancreatic cancer using PACO28238 at dilution of 1:100.
Immunofluorescent analysis of HepG2 cells using PACO28238 at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Background:
Nuclear monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin and regulates the SRF signaling. Acts by modifying nuclear actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Acts as a key regulator of the SRF signaling pathway elicited by nerve growth factor and serum: mediates oxidation and subsequent depolymerization of nuclear actin, leading to increase MKL1/MRTF-A presence in the nucleus and promote SRF:MKL1/MRTF-A-dependent gene transcription. Does not activate SRF:MKL1/MRTF-A through RhoA.
Synonyms:
[F-actin]-monooxygenase MICAL2 (EC 1.14.13.225) (Molecule interacting with CasL protein 2) (MICAL-2), MICAL2, KIAA0750 MICAL2PV1 MICAL2PV2
UniProt Protein Function:
MICAL2: Monooxygenase that promotes depolymerization of F-actin by mediating oxidation of specific methionine residues on actin. Acts by modifying actin subunits through the addition of oxygen to form methionine-sulfoxide, leading to promote actin filament severing and prevent repolymerization. Belongs to the Mical family. 6 isoforms of the human protein are produced by alternative splicing.Protein type: EC 1.14.13.-; Adaptor/scaffoldChromosomal Location of Human Ortholog: 11p15.3Cellular Component: nucleusMolecular Function: actin binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygenBiological Process: actin filament depolymerization; cytoskeleton organization and biogenesis; heart development; heart looping; sulfur oxidation
