The MIB1 Polyclonal Antibody (CAB8588) is a specialized tool for researchers studying the MIB1 protein, a marker of cell proliferation commonly used in cancer research and diagnosis. This antibody, generated in rabbits, has high specificity for human samples and has been validated for use in techniques such as immunohistochemistry and immunofluorescence.MIB1, also known as Ki-67, is a nuclear protein that is present in actively dividing cells, making it a reliable biomarker for assessing cellular proliferation in tissues. Its expression levels can provide valuable insights into the growth rate and aggressiveness of tumors, aiding in the diagnosis and prognosis of cancer patients.
By utilizing the MIB1 Polyclonal Antibody, researchers can confidently detect and quantify MIB1 expression in various tissue samples, facilitating their investigations into cell proliferation, tumor growth, and response to therapy. Its versatility and reliability make it an essential tool for advancing our understanding of cancer biology and improving patient care.
Product Name:
MIB1/DIP1 Rabbit Polyclonal Antibody
SKU:
CAB8588
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1/DIP1 (NP_065825.1).
This gene encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1).
Purification Method:
Affinity purification
Gene ID:
57534
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MIB1/DIP1 antibody (CAB8588) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
