MHC class I (HLA-A/B) Rabbit Monoclonal Antibody (CAB8754)
The MHC Class I Rabbit Monoclonal Antibody (CAB8754) is a cutting-edge tool for researchers studying MHC Class I molecules, important players in the immune system's recognition of foreign invaders. This antibody, generated in rabbits, demonstrates high specificity and sensitivity in detecting MHC Class I proteins in a variety of samples. Validated for use in various applications including Western blotting, this antibody enables precise and accurate analysis of MHC Class I expression in different cell types.MHC Class I molecules play a crucial role in presenting antigens to cytotoxic T cells, initiating immune responses against infected or abnormal cells.
By targeting and binding to MHC Class I proteins, this antibody allows researchers to investigate the role of these molecules in immune surveillance, viral infections, and autoimmune diseases. Understanding the function and regulation of MHC Class I molecules is essential for developing novel immunotherapies and vaccines targeted towards enhancing immune responses or preventing immune-related disorders.
Product Name:
MHC class I (HLA-A/B) Rabbit Monoclonal Antibody
SKU:
CAB8754
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MHC class I (HLA-A/B) (P04439).
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
3105/3106
Clone Number:
ARC1291
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MHC class I (HLA-A/B) Rabbit mAb (CAB8754) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
