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MHC class I (HLA-A/B) Rabbit Monoclonal Antibody (CAB8754)

SKU:
CAB8754
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
€139 - €419
Frequently bought together:

Description

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Product Name:MHC class I (HLA-A/B) Rabbit Monoclonal Antibody
SKU:CAB8754
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MHC class I (HLA-A/B) (P04439).
Sequence:KETL QRTD PPKT HMTH HPIS DHEA TLRC WALG FYPA EITL TWQR DGED QTQD TELV ETRP AGDG TFQK WAAV VVPS GEEQ RYTC HVQH EGLP KPLT LRWE L
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:HLAA; MHC class I (HLA-A/B)
Positive Sample:HeLa,HepG2,MCF7,Jurkat
Conjugate:Unconjugated
Calculated MW:41kDa
Observed MW:44kDa

HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008]

Purification Method:Affinity purification
Gene ID:3105/3106
Clone Number:ARC1291
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using MHC class I (HLA-A/B) Rabbit mAb (CAB8754) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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