The MHC Class 1 Rabbit Monoclonal Antibody is a powerful tool for researchers studying the major histocompatibility complex (MHC) class I molecules, which play a key role in presenting antigens to T cells and initiating immune responses. This monoclonal antibody, generated in rabbits, is highly specific and sensitive for detecting MHC class I in various samples, particularly in Western blot applications.The MHC Class 1 Rabbit Monoclonal Antibody binds specifically to MHC class I molecules, facilitating their visualization and analysis in different cell types and tissues.
This makes it an essential reagent for immunology studies, vaccine development, and investigations into infectious diseases and autoimmune disorders.Given the crucial role of MHC class I molecules in immune recognition and response, understanding their expression and function is essential for advancing research in immunotherapy, transplantation, and host-pathogen interactions. The MHC Class 1 Rabbit Monoclonal Antibody provides researchers with a reliable tool to explore the complexities of MHC class I biology and its implications for human health and disease.
Product Name:
HLA-A Rabbit Monoclonal Antibody
SKU:
CAB11406
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-A (P04439).
Cell membrane, Single-pass type I membrane protein, Endoplasmic reticulum membrane
Calculated MW:
41kDa
Observed MW:
41kDa
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.
Purification Method:
Affinity purification
Gene ID:
3105
Clone Number:
ARC0588
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using HLA-A Rabbit mAb (CAB11406) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
