The MGMT Rabbit Monoclonal Antibody is a powerful tool for research involving MGMT, a critical DNA repair enzyme that plays a key role in resistance to chemotherapy. This antibody, derived from rabbits, exhibits high specificity and sensitivity in detecting MGMT protein expression in human samples, making it an essential tool for studying drug resistance mechanisms in cancer cells.MGMT is involved in repairing DNA damage caused by alkylating agents, a common type of chemotherapy drug. Overexpression of MGMT in cancer cells can lead to resistance to these drugs, making it crucial to study its expression levels in tumors.
The MGMT Rabbit Monoclonal Antibody specifically binds to the MGMT protein, allowing for precise detection and quantification in various cancer cell lines and tissue samples.Research into MGMT expression and activity is vital for developing new strategies to overcome chemoresistance in cancer treatment. By utilizing the MGMT Rabbit Monoclonal Antibody, researchers can gain a deeper understanding of how MGMT influences drug response and develop more effective therapies for patients with resistant tumors.
Product Name:
MGMT Rabbit Monoclonal Antibody
SKU:
CAB11151
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 108-207 of human MGMT (NP_002403.3).
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Purification Method:
Affinity purification
Gene ID:
4255
Clone Number:
ARC0529
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MGMT Rabbit mAb (CAB11151) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
