The MGMT Polyclonal Antibody (CAB0693) is a valuable tool for researchers studying the MGMT protein, which plays a key role in DNA repair mechanisms and resistance to chemotherapeutic agents. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By binding to the MGMT protein, it allows for accurate detection and analysis, making it ideal for studies in oncology and drug resistance research.
MGMT, also known as O-6-methylguanine-DNA methyltransferase, is a critical enzyme involved in protecting cells from DNA damage caused by alkylating agents. Its role in DNA repair pathways makes it a significant target for understanding mechanisms of drug resistance in cancer treatment. Studies on MGMT expression and activity are essential for developing strategies to overcome resistance and improve the efficacy of chemotherapy in cancer patients.
Product Name:
MGMT Rabbit Polyclonal Antibody
SKU:
CAB0693
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (NP_002403.2).
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Purification Method:
Affinity purification
Gene ID:
4255
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using MGMT antibody (CAB0693) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
