The MGMT Polyclonal Antibody (CAB0052) is a valuable tool for researchers studying the DNA repair enzyme O-6-Methylguanine-DNA Methyltransferase (MGMT). This antibody, produced in rabbits, is highly specific and sensitive for detecting MGMT in human samples, making it an essential component for Western blot applications.MGMT plays a crucial role in protecting cells from DNA damage caused by alkylating agents, making it a key player in cancer research and chemotherapy resistance studies.
By targeting MGMT, researchers can better understand mechanisms of drug resistance and develop more effective cancer treatments.With its high reactivity and specificity, the MGMT Polyclonal Antibody (CAB0052) is an essential tool for studying MGMT expression and function in various cell types. Its versatility and reliability make it an indispensable resource for advancing research in oncology and drug development.
Product Name:
MGMT Rabbit Polyclonal Antibody
SKU:
CAB0052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human MGMT (NP_002403.2).
Alkylating agents are potent carcinogens that can result in cell death, mutation and cancer. The protein encoded by this gene is a DNA repair protein that is involved in cellular defense against mutagenesis and toxicity from alkylating agents. The protein catalyzes transfer of methyl groups from O(6)-alkylguanine and other methylated moieties of the DNA to its own molecule, which repairs the toxic lesions. Methylation of the genes promoter has been associated with several cancer types, including colorectal cancer, lung cancer, lymphoma and glioblastoma.
Purification Method:
Affinity purification
Gene ID:
4255
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using MGMT antibody (CAB0052) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 30s.
