The MGAT5 Antibody (CAB10567) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in various research applications such as Western blotting.MGAT5, also known as beta-1,6-N-acetylglucosaminyltransferase, plays a crucial role in the modification of glycoproteins, impacting cellular processes such as cell-cell interactions, cell signaling, and immune response. Dysregulation of MGAT5 expression has been linked to various diseases including cancer, diabetes, and autoimmune disorders, highlighting its potential as a therapeutic target.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse samples.
Product Name:
MGAT5 Antibody
SKU:
CAB10567
Size:
20μL, 100μL
Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
GNT-V, GNT-VA, MGAT5A, glcNAc-T V, GNT-V/MGAT5
Positive Sample:
SH-SY5Y, Jurkat, Mouse testis, Mouse thymus
Cellular Localization:
Golgi Apparatus Membrane, Single-Pass Type Ii Membrane Protein.
Calculated MW:
85kDa
Observed MW:
100kDa
The protein encoded by this gene belongs to the glycosyltransferase family. It catalyzes the addition of beta-1,6-N-acetylglucosamine to the alpha-linked mannose of biantennary N-linked oligosaccharides present on the newly synthesized glycoproteins. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. Alterations of the oligosaccharides on cell surface glycoproteins cause significant changes in the adhesive or migratory behavior of a cell. Increase in the activity of this enzyme has been correlated with the progression of invasive malignancies.
Purification Method
Affinity purification
Gene ID
4249
RRID
AB_2758108
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using GNT-V/MGAT5 Rabbit pAb (CAB10567) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 180s.
Western blot analysis of various lysates using GNT-V/MGAT5 Rabbit pAb (CAB10567) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 90s.
