The MGAT1 Polyclonal Antibody (CAB6387) is a valuable tool for researchers studying the MGAT1 protein, an enzyme involved in the biosynthesis of complex N-glycans. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to MGAT1, researchers can effectively detect and analyze this glycosyltransferase in a variety of cell types.MGAT1 plays a crucial role in the modification of glycoproteins, impacting cell signaling, adhesion, and immune responses.
Dysregulation of MGAT1 has been implicated in various diseases, including cancer, diabetes, and autoimmune disorders. The ability to study MGAT1 using this polyclonal antibody provides valuable insights for research in glycobiology, immunology, and disease mechanisms. Understanding the function of MGAT1 could lead to the development of targeted therapies for a range of illnesses characterized by glycan abnormalities.
Product Name:
MGAT1 Rabbit Polyclonal Antibody
SKU:
CAB6387
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 156-445 of human MGAT1 (NP_001108092.1).
Golgi apparatus membrane, Single-pass type II membrane protein.
Calculated MW:
51kDa
Observed MW:
60kDa
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis. Several variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4245
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using MGAT1 Rabbit pAb (CAB6387) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
