The MFNG Polyclonal Antibody (PAC062943) is a valuable tool for researchers studying the MFNG protein, a critical regulator of Notch signaling pathways. This antibody, produced in rabbits, boasts high reactivity with human samples and is validated for use in Western blot applications. By targeting the MFNG protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential component of studies in developmental biology and cancer research.MFNG, also known as O-fucosylpeptide 3-β-N-acetylglucosaminyltransferase, plays a crucial role in modulating Notch signaling, a pathway involved in cell differentiation, proliferation, and apoptosis.
Aberrant Notch signaling has been implicated in various cancers and developmental disorders, highlighting the importance of understanding and manipulating MFNG activity. By studying the function of MFNG, researchers can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets for a range of conditions.
Antibody Name:
MFNG Antibody (PACO62943)
Antibody SKU:
PACO62943
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human Beta-1,3-N-acetylglucosaminyltransferase manic fringe protein (28-321AA)
IHC image of PACO62943 diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity.
MFNG: Glycosyltransferase involved in the elongation of O- linked ligands to activate Notch signaling. Possesses fucose- specific beta-1,3-N-acetylglucosaminyltransferase activity. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Transferase; Membrane protein, integral; EC 2.4.1.222Chromosomal Location of Human Ortholog: 22q12Cellular Component: extracellular space; integral to Golgi membraneMolecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activityBiological Process: positive regulation of protein binding; metabolic process; pattern specification process; positive regulation of Notch signaling pathway
UniProt Protein Details:
NCBI Summary:
This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]
