The MFNG Polyclonal Antibody (PACO22197) is a valuable tool for researchers studying the MFNG protein, which is involved in various cellular processes including Notch signaling and glycosylation. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding specifically to the MFNG protein, this antibody enables researchers to detect and analyze MFNG expression in a wide range of cell types.MFNG is a critical regulator of Notch signaling, a pathway known for its role in cell fate determination and development. Dysregulation of Notch signaling has been implicated in various diseases, making MFNG an important target for research in fields such as cancer biology and developmental biology.
By understanding the function of MFNG, researchers can gain insights into its potential therapeutic implications in diseases where Notch signaling is dysregulated.Overall, the MFNG Polyclonal Antibody (PACO22197) is a valuable tool for researchers looking to explore the role of MFNG in cellular processes and disease mechanisms. Its specificity and reliability make it an ideal choice for studies in molecular biology, cancer research, and developmental biology.
Antibody Name:
MFNG Antibody (PACO22197)
Antibody SKU:
PACO22197
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human MFNG.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HepG2 cells, using MFNG antibody.
Background:
Glycosyltransferase involved in the elongation of O-linked ligands to activate Notch signaling. Possesses fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity.
MFNG: Glycosyltransferase involved in the elongation of O- linked ligands to activate Notch signaling. Possesses fucose- specific beta-1,3-N-acetylglucosaminyltransferase activity. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Transferase; Membrane protein, integral; EC 2.4.1.222Chromosomal Location of Human Ortholog: 22q12Cellular Component: extracellular space; integral to Golgi membraneMolecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activityBiological Process: positive regulation of protein binding; metabolic process; pattern specification process; positive regulation of Notch signaling pathway
UniProt Protein Details:
NCBI Summary:
This gene is a member of the glycosyltransferase 31 gene family. Members of this gene family, which also includes the LFNG (GeneID: 3955) and RFNG (GeneID: 5986) genes, encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, these proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. The protein encoded by this gene may control Notch signaling in claudin-low breast cancer. [provided by RefSeq, May 2018]
