The MEK1 Rabbit Monoclonal Antibody (CAB19565) is an essential tool for researchers studying the MEK1 protein, a key player in the MAPK/ERK signaling pathway involved in cell proliferation, differentiation, and survival. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting MEK1 in human samples, making it suitable for Western blot applications.MEK1 is a critical component of the MAPK/ERK pathway, which is often dysregulated in cancer, making it a promising target for therapeutic interventions. By targeting MEK1, researchers can gain valuable insights into the mechanisms underlying tumorigenesis and potentially develop novel treatment strategies for various types of cancer.
With its ability to accurately detect and analyze MEK1 protein levels in different cell types, the MEK1 Rabbit Monoclonal Antibody is a valuable tool for research in oncology and drug development. By understanding the role of MEK1 in disease progression, researchers can pave the way for more effective targeted therapies and personalized medicine approaches in the future.
Product Name:
MEK1 Rabbit Monoclonal Antibody
SKU:
CAB19565
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MEK1 (Q02750).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Synonyms:
MEL; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
Positive Sample:
HeLa,HepG2,A-431,Mouse brain,Mouse lung,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Membrane, Nucleus, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center, spindle pole body.
Calculated MW:
43kDa
Observed MW:
45kDa
The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604
Clone Number:
ARC0044
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using MEK1 Rabbit mAb (CAB19565) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
