The MEK1/MEK2 Polyclonal Antibody (CAB18117) is a valuable tool for researchers studying the MEK1 and MEK2 proteins, key players in the MAPK/ERK signaling pathway. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to MEK1 and MEK2, this antibody allows for the detection and analysis of these important signaling molecules in a variety of cell types.MEK1 and MEK2 are critical components of the MAPK/ERK pathway, which regulates cell growth, differentiation, and survival.
Dysregulation of this pathway is commonly observed in cancer and other diseases, making MEK1 and MEK2 attractive targets for therapeutic intervention. The MEK1/MEK2 Polyclonal Antibody offers researchers a reliable tool for studying the function and activity of these proteins in the context of various physiological and pathological processes.
Product Name:
MEK1/MEK2 Rabbit Polyclonal Antibody
SKU:
CAB18117
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human MAP2K1 (NP_002746.1).
cytosol, early endosome, endoplasmic reticulum, focal adhesion, Golgi apparatus, late endosome, microtubule organizing center, mitochondrion, nucleus, plasma membrane
Calculated MW:
40kDa/43kDa/44kDa
Observed MW:
44kDa
The protein MEK1 and MEK2 are two members of the dual specificity protein kinase family, which act as mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.
Purification Method:
Affinity purification
Gene ID:
5604/5605
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of PC-12 cells, using MAP2K1/MAP2K2 antibody (CAB18117) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
