The MEK (Methionine Enkephalin) ELISA Kit is specifically designed for the precise detection of Methionine Enkephalin levels in various biological samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an ideal tool for a wide range of research applications.Methionine Enkephalin is a neuropeptide that plays a crucial role in pain modulation, neuroprotection, and immune regulation.
Its levels have been implicated in various physiological and pathological conditions, making it a valuable biomarker for studying these conditions and exploring potential therapeutic interventions.Overall, the MEK ELISA Kit offers researchers a reliable and efficient way to measure Methionine Enkephalin levels, advancing our understanding of its roles in health and disease.
Product Name:
MEK (Methionine-Enkephalin) ELISA Kit (UNES00015)
SKU:
UNES00015
Target:
MEK (Methionine-Enkephalin) (UNES00015)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
75.00 pg/mL
Detection range:
125.00-8000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with MEK. During the reaction, MEK in the sample or standard competes with a fixed amount of MEK on the solid phase supporter for sites on the Biotinylated Detection Ab specific to MEK. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MEK in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
98-114
89-102
Average (%)
91
105
94
1:4
Range (%)
84-99
99-112
84-95
Average (%)
90
104
89
1:8
Range (%)
87-100
96-108
87-97
Average (%)
93
102
92
1:16
Range (%)
86-100
99-115
84-98
Average (%)
92
105
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
99
EDTA plasma (n=5)
92-106
99
Cell culture media (n=5)
92-105
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
440.94
1081.84
3418.41
427.7
1013.67
3096.13
Standard deviation
22.66
56.69
141.52
27.33
58.59
117.34
C V (%)
5.14
5.24
4.14
6.39
5.78
3.79
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of MEK concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes MEK in samples. No significant cross-reactivity or interference between MEK and analogues was observed.
