The MEGF8 Polyclonal Antibody (PACO59193) is a valuable tool for researchers studying MEGF8, a protein involved in key cellular processes such as cell adhesion and signaling. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot applications. By targeting the MEGF8 protein, this antibody allows for precise detection and analysis in various cell types, making it ideal for investigations in developmental biology and neurology.MEGF8 is known to play a crucial role in neuronal development and synaptic function, making it a promising target for studies on neurological disorders and neurodevelopmental diseases.
Its involvement in cellular processes related to cell adhesion and signaling highlights its potential significance in understanding various aspects of brain development and function. Researchers studying MEGF8 can gain valuable insights into the molecular mechanisms underlying neurological conditions and explore potential therapeutic strategies targeting this protein.
IHC image of PACO59193 diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of HepG2 cells with PACO59193 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Multiple epidermal growth factor-like domains protein 8 (Multiple EGF-like domains protein 8) (Epidermal growth factor-like protein 4) (EGF-like protein 4), MEGF8, C19orf49 EGFL4 KIAA0817
UniProt Protein Function:
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene is a single-pass type I membrane protein of unknown function that contains several EGF-like domains, Kelch repeats, and PSI domains. Defects in this gene are a cause of Carpenter syndrome 2. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]
