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MEF2B Colorimetric Cell-Based ELISA

SKU:
CBCAB01058
Product Type:
ELISA Kit
ELISA Type:
Cell Based
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human
Mouse
Detection Method:
Colorimetric
$719
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Description

system_update_altDatasheetMSDS

MEF2B Colorimetric Cell-Based ELISA

The MEF2B Colorimetric Cell-Based ELISA Kit is a powerful and innovative tool for studying the role of MEF2B in cellular signaling pathways. This kit allows for the accurate and reliable detection of MEF2B levels in cell lysates, making it ideal for researchers conducting experiments in cell biology, molecular biology, and related fields.MEF2B is a transcription factor that plays a critical role in the regulation of gene expression, cell differentiation, and apoptosis. Dysregulation of MEF2B has been implicated in various diseases, including cancer, cardiovascular diseases, and neurological disorders, making it an important target for drug development and therapeutic interventions.

With its high sensitivity and specificity, the MEF2B Colorimetric Cell-Based ELISA Kit provides researchers with a valuable tool for studying the function and regulation of MEF2B in cellular processes. Its ease of use and reproducibility make it a reliable choice for a wide range of research applications. Upgrade your research capabilities with the MEF2B Colorimetric Cell-Based ELISA Kit today.

Product Name:MEF2B Colorimetric Cell-Based ELISA
Product Code:CBCAB01058
ELISA Type:Cell-Based
Target:MEF2B
Reactivity:Human, Mouse
Dynamic Range:> 5000 Cells
Detection Method:Colorimetric 450 nmStorage/Stability:4°C/6 Months
Format:96-Well Microplate

The MEF2B Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect MEF2B protein expression profile in cells. The kit can be used for measuring the relative amounts of MEF2B in cultured cells as well as screening for the effects that various treatments, inhibitors (ie siRNA or chemicals), or activators have on MEF2B.

Qualitative determination of MEF2B concentration is achieved by an indirect ELISA format. In essence, MEF2B is captured by MEF2B-specific primary antibodies while the HRP-conjugated secondary antibodies bind the Fc region of the primary antibody. Through this binding, the HRP enzyme conjugated to the secondary antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are needed:

1. A monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values.
2. Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method may be used to determine cell density. After staining, the results can be analysed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Database Information:Gene ID: 4207, UniProt ID: Q02080, OMIM: 600661, Unigene: Hs.153629
Gene Symbol:MEF2B
Sub Type:None
UniProt Protein Function:MEF2B: Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle- specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription. Belongs to the MEF2 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.11

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cell junction; nucleus

Molecular Function:protein dimerization activity; histone deacetylase binding; transcription factor activity

Biological Process: muscle development; transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter

NCBI Summary:This gene represents numerous read-through transcripts that span GeneID:729991 and 100271849. Many read-through transcripts are predicted to be nonsense-mediated decay (NMD) candidates, and are thought to be non-coding. Some transcripts are predicted to be capable of translation reinitiation at a downstream AUG, resulting in expression of at least one isoform of myocyte enhancer factor 2B (MEF2B) from this read-through locus. At least one additional MEF2B variant and isoform can be expressed from a downstream promoter, and is annotated on GeneID:100271849. [provided by RefSeq, Oct 2010]
UniProt Code:Q02080
NCBI GenInfo Identifier:5174543
NCBI Gene ID:4207
NCBI Accession:NP_005910
UniProt Related Accession:Q02080
Molecular Weight:39kDa
NCBI Full Name:myocyte-specific enhancer factor 2B isoform b
NCBI Synonym Full Names:BORCS8-MEF2B readthrough
NCBI Official Symbol:BORCS8-MEF2B  
NCBI Official Synonym Symbols:MEF2B; RSRFR2; MEF2BNB-MEF2B; LOC729991-MEF2B  
NCBI Protein Information:myocyte-specific enhancer factor 2B
UniProt Protein Name:Myocyte-specific enhancer factor 2B
UniProt Synonym Protein Names:RSRFR2; Serum response factor-like protein 2
Protein Family:Myocyte-specific enhancer factor
UniProt Gene Name:MEF2B  
UniProt Entry Name:MEF2B_HUMAN
ComponentQuantity
96-Well Cell Culture Clear-Bottom Microplate2 plates
10X TBS24 mL
Quenching Buffer24 mL
Blocking Buffer50 mL
15X Wash Buffer50 mL
Primary Antibody Diluent12 mL
100x Anti-Phospho Target Antibody 60 µL
100x Anti-Target Antibody60 µL
Anti-GAPDH Antibody60 µL
HRP-Conjugated Anti-Rabbit IgG Antibody12 mL
HRP-Conjugated Anti-Mouse IgG Antibody12 mL
SDS Solution12 mL
Stop Solution24 mL
Ready-to-Use Substrate12 mL
Crystal Violet Solution12 mL
Adhesive Plate Seals2 seals

The following materials and/or equipment are NOT provided in this kit but are necessary to successfully conduct the experiment:

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 µL to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing ≥1 ml
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
  • Orbital shaker (optional)
  • Deionized or sterile water

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

StepProcedure
1.Seed 200 µL of 20,000 adherent cells in culture medium in each well of a 96-well plate. The plates included in the kit are sterile and treated for cell culture. For suspension cells and loosely attached cells, coat the plates with 100 µL of 10 µg/ml Poly-L-Lysine (not included) to each well of a 96-well plate for 30 minutes at 37°C prior to adding cells.
2.Incubate the cells for overnight at 37°C, 5% CO2.
3.Treat the cells as desired.
4.Remove the cell culture medium and rinse with 200 µL of 1x TBS, twice.
5.Fix the cells by incubating with 100 µL of Fixing Solution for 20 minutes at room temperature. The 4% formaldehyde is used for adherent cells and 8% formaldehyde is used for suspension cells and loosely attached cells.
6.Remove the Fixing Solution and wash the plate 3 times with 200 µL 1x Wash Buffer for five minutes each time with gentle shaking on the orbital shaker. The plate can be stored at 4°C for a week.
7.Add 100 µL of Quenching Buffer and incubate for 20 minutes at room temperature.
8.Wash the plate 3 times with 1x Wash Buffer for 5 minutes each time.
9.Add 200 µL of Blocking Buffer and incubate for 1 hour at room temperature.
10. Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
11.Add 50 µL of 1x primary antibodies (Anti-MEF2B Antibody and/or Anti-GAPDH Antibody) to the corresponding wells, cover with Parafilm and incubate for 16 hours (overnight) at 4°C. If the target expression is known to be high, incubate for 2 hours at room temperature.
12.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
13.Add 50 µL of 1x secondary antibodies (HRP-Conjugated AntiRabbit IgG Antibody or HRP-Conjugated Anti-Mouse IgG Antibody) to corresponding wells and incubate for 1.5 hours at room temperature.
14.Wash 3 times with 200 µL of 1x Wash Buffer for 5 minutes each time.
15.Add 50 µL of Ready-to-Use Substrate to each well and incubate for 30 minutes at room temperature in the dark.
16.Add 50 µL of Stop Solution to each well and read OD at 450 nm immediately using the microplate reader.

(Additional Crystal Violet staining may be performed if desired – details of this may be found in the kit technical manual.)

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