The Med8 Antibody (PACO02420) is a high-quality polyclonal antibody specifically designed for research involving the Med8 protein. Med8 is a subunit of the Mediator complex, a key regulator of transcription that plays a crucial role in gene expression. This antibody is raised in rabbits and is highly reactive with human samples, making it an ideal tool for researchers studying transcriptional regulation and gene expression.Validated for use in Western blot applications, the Med8 Antibody binds specifically to the Med8 protein, allowing for the detection and analysis of Med8 in various cell types and tissues. Its specificity and sensitivity make it a valuable asset for studies in molecular biology, genetics, and biochemistry, providing insights into the mechanisms underlying gene transcription and regulation.
The Med8 Antibody is essential for understanding the function of the Mediator complex and its role in controlling gene expression in health and disease. By studying Med8 and its interactions with other subunits of the complex, researchers can gain valuable insights into the mechanisms that drive cellular processes and potentially identify new targets for therapeutic intervention in various diseases, including cancer and metabolic disorders.
Antibody Name:
MED8 Antibody
Antibody SKU:
PACO02420
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:2000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from the Internal region of human Med8.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MED8; Mediator of RNA polymerase II transcription subunit 8; Activator-recruited cofactor 32 kDa component; ARC32; Mediator complex subunit 8
UniProt Protein Function:
MED8: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May play a role as a target recruitment subunit in E3 ubiquitin-protein ligase complexes and thus in ubiquitination and subsequent proteasomal degradation of target proteins. Belongs to the Mediator complex subunit 8 family. 3 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 1p34.2Cellular Component: nucleoplasm; Srb-mediator complexMolecular Function: protein bindingBiological Process: transcription initiation from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jan 2013]
