The MED27 Polyclonal Antibody (PAC037146) is a valuable tool for researchers studying the MED27 protein, a subunit of the Mediator complex involved in transcriptional regulation. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. By targeting the MED27 protein, this antibody enables the detection and analysis of MED27 expression in various cell types, making it an essential reagent for studies in molecular biology and gene regulation.
MED27 is a key player in gene expression regulation, acting as a bridge between transcription factors and RNA polymerase II. Its role in mediating transcriptional activation makes it an attractive target for research into various diseases, including cancer and developmental disorders. Understanding the function of MED27 is essential for uncovering the mechanisms underlying these pathologies and developing targeted therapies to address them.
Antibody Name:
MED27 Antibody (PACO37146)
Antibody SKU:
PACO37146
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:5000
Species Reactivity:
Human
Immunogen:
Recombinant Human Mediator of RNA polymerase II transcription subunit 27 protein (39-108AA)
Western blot. All lanes: MED27 antibody at 0.5µg/ml + A431 whole cell lysate. Secondary. Goat polyclonal to rabbit IgG at 1/10000 dilution. Predicted band size: 36, 32, 15 kDa. Observed band size: 36 kDa.
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 9q34.13Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; transcription factor complex; ubiquitin ligase complexMolecular Function: protein binding; transcription coactivator activityBiological Process: gene expression; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; transcription initiation from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
