The MED27 Polyclonal Antibody (PAC02715) is a valuable tool for researchers studying the MED27 protein, a subunit of the Mediator complex involved in transcription regulation. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to MED27, it allows for the detection and analysis of this important protein in various cell types, making it an ideal choice for studies in molecular biology and gene regulation.The MED27 protein is a key player in gene expression control, functioning as a bridge between transcription factors and RNA polymerase II. Understanding its role in transcriptional regulation is essential for unraveling the complexities of gene regulation and cellular processes.
Research on MED27 is crucial for insights into diseases related to dysregulated gene expression, such as cancer, genetic disorders, and developmental abnormalities.By using the MED27 Polyclonal Antibody, researchers can further investigate the functions and mechanisms of MED27 in various biological contexts, paving the way for potential therapeutic interventions targeting gene expression pathways. Its high specificity and sensitivity make it a reliable tool for exploring the intricate networks of gene regulation and transcriptional control.
Antibody Name:
MED27 Antibody (PACO22715)
Antibody SKU:
PACO22715
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from Internal of human MED27.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, using MED27 antibody.
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Synonyms:
Cofactor required for Sp1 transcriptional activation subunit 8; cofactor required for Sp1 transcriptional activation; subunit 8 (34kD); cofactor required for Sp1 transcriptional activation; subunit 8
UniProt Protein Function:
MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 9q34.13Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; transcription factor complex; ubiquitin ligase complexMolecular Function: protein binding; transcription coactivator activityBiological Process: gene expression; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; transcription initiation from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
