MED27 Antibody (PACO22715)
- SKU:
- PACO22715
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Antibody Name: | MED27 Antibody (PACO22715) |
| Antibody SKU: | PACO22715 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:3000 |
| Species Reactivity: | Human, Mouse |
| Immunogen: | Synthesized peptide derived from Internal of human MED27. |
| Form: | Liquid |
| Storage Buffer: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from HeLa cells, using MED27 antibody. |
| Background: | Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. |
| Synonyms: | Cofactor required for Sp1 transcriptional activation subunit 8; cofactor required for Sp1 transcriptional activation; subunit 8 (34kD); cofactor required for Sp1 transcriptional activation; subunit 8 |
| UniProt Protein Function: | MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 9q34.13Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; transcription factor complex; ubiquitin ligase complexMolecular Function: protein binding; transcription coactivator activityBiological Process: gene expression; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; transcription initiation from RNA polymerase II promoter |
| UniProt Protein Details: | |
| NCBI Summary: | The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q6P2C8 |
| NCBI GenInfo Identifier: | 74737195 |
| NCBI Gene ID: | 9442 |
| NCBI Accession: | Q6P2C8.1 |
| UniProt Secondary Accession: | Q6P2C8,O95401, Q4F964, Q5VTA4, Q5VTA5, Q9BU57, Q9NYR4 V9GYV9 |
| UniProt Related Accession: | Q6P2C8 |
| Molecular Weight: | 14,826 Da |
| NCBI Full Name: | Mediator of RNA polymerase II transcription subunit 27 |
| NCBI Synonym Full Names: | mediator complex subunit 27 |
| NCBI Official Symbol: | MED27Â Â |
| NCBI Official Synonym Symbols: | MED3; CRSP8; CRAP34; CRSP34; TRAP37Â Â |
| NCBI Protein Information: | mediator of RNA polymerase II transcription subunit 27 |
| UniProt Protein Name: | Mediator of RNA polymerase II transcription subunit 27 |
| UniProt Synonym Protein Names: | Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional coactivator CRSP34 |
| Protein Family: | Mediator of RNA polymerase II transcription |
| UniProt Gene Name: | MED27Â Â |
| UniProt Entry Name: | MED27_HUMAN |
| Antibodies |
| MED27 Antibody (PACO05767) |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
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