The MED27 Polyclonal Antibody (PACO05767) is a valuable tool for researchers studying the MED27 protein, which plays a critical role in transcription regulation and gene expression. Raised in rabbits, this antibody has high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the MED27 protein, researchers can accurately detect and analyze its presence in various cell types, making it ideal for studies in molecular biology and genetics.MED27 is a key component of the Mediator complex, a vital mediator of transcriptional regulation that links transcription factors to RNA polymerase II.
Dysregulation of MED27 has been implicated in various diseases, including cancer, cardiovascular disorders, and developmental abnormalities. Understanding the function of MED27 is essential for unraveling the mechanisms underlying these conditions and identifying potential therapeutic targets.By utilizing the MED27 Polyclonal Antibody, researchers can delve deeper into the role of MED27 in transcriptional regulation and gene expression, paving the way for a better understanding of disease mechanisms and the development of targeted interventions.
Antibody Name:
MED27 Antibody (PACO05767)
Antibody SKU:
PACO05767
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:10000, WB:1:500-1:2000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from the Internal region of human CRSP34.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
MED27; CRSP34; CRSP8; Mediator of RNA polymerase II transcription subunit 27; Cofactor required for Sp1 transcriptional activation subunit 8; CRSP complex subunit 8; Mediator complex subunit 27; P37 TRAP/SMCC/PC2 subunit; Transcriptional co
UniProt Protein Function:
MED27: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 27 family. 2 isoforms of the human protein are produced by alternative splicing.Chromosomal Location of Human Ortholog: 9q34.13Cellular Component: cytoplasm; nucleolus; nucleoplasm; nucleus; Srb-mediator complex; transcription factor complex; ubiquitin ligase complexMolecular Function: protein binding; transcription coactivator activityBiological Process: gene expression; protein ubiquitination; regulation of transcription from RNA polymerase II promoter; stem cell maintenance; transcription initiation from RNA polymerase II promoter
UniProt Protein Details:
NCBI Summary:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]
