The MED26 Antibody (PAC021710) is a vital tool for research involving MED26, a key component of the mediator complex that plays a crucial role in transcription regulation. This polyclonal antibody, generated in rabbits, exhibits high reactivity with human samples and has been extensively validated for use in Western blot applications. It specifically binds to the MED26 protein, enabling accurate detection and analysis in various cell types, making it an invaluable asset for studies in molecular biology and gene regulation.MED26, also known as ARC, is essential for the assembly and function of the mediator complex, which serves as a bridge between transcription factors and the RNA polymerase II machinery, facilitating the activation of gene expression.
Dysregulation of MED26 has been linked to various diseases such as cancer, developmental disorders, and neurological conditions, highlighting its importance in cellular function and human health. Investigating the role of MED26 is critical for advancing our understanding of gene regulation mechanisms and identifying potential therapeutic targets for disease intervention.
Antibody Name:
MED26 Antibody (PACO21710)
Antibody SKU:
PACO21710
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human, Mouse
Immunogen:
Synthesized peptide derived from N-terminal of human MED26.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from LOVO cells, using MED26 antibody.
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Naeaer A.M., Nature 398:828-832(1999).
MED26: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 26 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:
Protein type:Transcription, coactivator/corepressor
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
NCBI Summary:
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. [provided by RefSeq, Jul 2008]
