The MED25 Polyclonal Antibody (CAB16150) is a valuable tool for researchers studying the MED25 protein, a transcriptional coactivator known for its role in gene regulation and cellular signaling pathways. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it an excellent choice for Western blot applications. By specifically binding to the MED25 protein, this antibody enables the detection and analysis of MED25 in various cell types, providing essential insights for studies in molecular biology and cancer research.
MED25 is a key player in transcriptional regulation, playing a crucial role in the activation of gene expression programs that control a variety of cellular processes. Dysregulation of MED25 has been implicated in various diseases, including cancer and metabolic disorders, making it a promising target for therapeutic intervention. By understanding the function and regulation of MED25, researchers can uncover new pathways for developing innovative treatments and personalized medicine approaches.
Product Name:
MED25 Rabbit Polyclonal Antibody
SKU:
CAB16150
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human MED25 (NP_112235.2).
This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
Purification Method:
Affinity purification
Gene ID:
81857
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MED25 Rabbit pAb (CAB16150) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
