The MED25 Polyclonal Antibody is a valuable tool for researchers studying the MED25 protein, a transcriptional coactivator involved in gene regulation and cell signaling pathways. This antibody, generated in rabbits, is highly specific for detecting MED25 in human samples and is validated for use in various applications, including immunofluorescence and immunoprecipitation.MED25 plays a crucial role in regulating gene expression and is known to interact with multiple transcription factors and other coactivators to modulate transcriptional activity. Research into MED25 is essential for understanding its function in various biological processes, such as cell growth, differentiation, and development.
With its high reactivity and specificity, the MED25 Polyclonal Antibody is a valuable tool for researchers conducting studies in molecular biology, cancer research, and gene regulation. By enabling the detection and analysis of MED25, this antibody provides insights into its role in various cellular processes, leading to a better understanding of its potential implications in disease development and progression.
Product Name:
Med25 Polyclonal Antibody
SKU:
CAB21274
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Arabidopsis thaliana
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of arabidopsis thaliana Med25 (NP_173925.3).
Encodes a nuclear protein that acts in a phyB pathway (but downstream of phyB) and induces flowering in response to suboptimal light conditions. PFT1 promotes flowering in CO dependent and independent pathways and integrates several environmental stimuli, such as light quality and JA-dependent defenses. Mutants are hypo-responsive to far-red and hyper-responsive to red light and flower late under long day conditions. Also shown to be a Mediator subunit regulating jasmonate-dependent defense.
Purification Method:
Affinity purification
Gene ID:
839141
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using Med25 Rabbit pAb (CAB21274) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
