The MED21 Polyclonal Antibody (CAB12168) is a valuable tool for researchers studying the MED21 protein, a subunit of the Mediator complex involved in transcriptional regulation. This antibody is produced in rabbits and exhibits high reactivity with human samples, making it suitable for use in Western blot applications. By specifically binding to the MED21 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for research in molecular biology and gene regulation.The MED21 protein is essential for the proper functioning of the Mediator complex, which plays a key role in mediating interactions between transcription factors and RNA polymerase II during gene expression.
Understanding the role of MED21 in transcriptional regulation is crucial for unraveling the complex mechanisms that control gene expression and cellular processes. Research involving the MED21 Polyclonal Antibody can provide valuable insights into the molecular pathways regulated by the Mediator complex, with implications for various fields such as cancer biology, developmental biology, and stem cell research.
Product Name:
MED21 Rabbit Polyclonal Antibody
SKU:
CAB12168
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human MED21 (NP_004255.2).
This gene encodes a member of the mediator complex subunit 21 family. The encoded protein interacts with the human RNA polymerase II holoenzyme and is involved in transcriptional regulation of RNA polymerase II transcribed genes. A pseudogene of this gene is located on chromosome 8. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
9412
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MED21 Rabbit pAb (CAB12168) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
