The MED20 Polyclonal Antibody (CAB15757) is a research tool designed for studying the MED20 protein, which plays a key role in mediating gene transcription. This antibody, produced in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the MED20 protein, this antibody allows for easy detection and analysis in a variety of cell types, making it an ideal tool for research in the fields of molecular biology and gene regulation.MED20 is a subunit of the mediator complex, which functions as a transcriptional coactivator to regulate gene expression. Its involvement in gene transcription makes it a crucial target for studying various cellular processes, including cell growth, differentiation, and response to stimuli.
Research on the MED20 protein is essential for advancing our understanding of gene regulation mechanisms and identifying potential therapeutic targets for diseases related to aberrant gene expression.Overall, the MED20 Polyclonal Antibody is a valuable tool for researchers interested in studying the role of the MED20 protein in gene transcription and exploring its potential implications for human health and disease. Its high reactivity and specificity make it a reliable choice for experiments aimed at unraveling the complexities of gene regulation pathways.
Product Name:
MED20 Rabbit Polyclonal Antibody
SKU:
CAB15757
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human MED20 (NP_004266.2).
This gene encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. A mutation in this gene has been associated with a novel infantile-onset neurodegenerative movement disorder. Alternatively spliced transcript variants have been identified.
Purification Method:
Affinity purification
Gene ID:
9477
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MED20 Rabbit pAb (CAB15757) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
