The MED17 Polyclonal Antibody (CAB8716) is a valuable tool for researchers studying the MED17 protein, a key component of the Mediator complex involved in gene transcription regulation. This antibody, raised in rabbits, exhibits high reactivity with MED17 in human samples and is validated for use in Western blot applications. By binding to the MED17 protein, this antibody enables accurate detection and analysis in various cell types, making it an essential asset for studies in molecular biology and gene regulation.
MED17, a subunit of the Mediator complex, plays a crucial role in coordinating communication between transcription factors and RNA polymerase II during gene expression. Dysregulation of MED17 has been linked to various diseases, including cancer and developmental disorders, highlighting its importance in cellular function. Research into the function and regulation of MED17 is vital for advancing our understanding of gene transcription mechanisms and developing targeted therapies for related pathologies.
Product Name:
MED17 Rabbit Polyclonal Antibody
SKU:
CAB8716
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-460 of human MED17 (NP_004259.3).
The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Purification Method:
Affinity purification
Gene ID:
9440
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MED17 Rabbit pAb (CAB8716) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
