The MED16 Polyclonal Antibody (PAC017236) is a vital tool for researchers studying the MED16 protein, a key component of the Mediator complex that regulates gene expression. This antibody, produced in rabbits, has high specificity for human samples and is suitable for use in Western blot applications. By targeting the MED16 protein, researchers can investigate its role in transcriptional regulation in various cell types, making it an essential tool for studies in molecular biology and gene expression analysis.
MED16 is a crucial mediator of transcriptional activation, playing a critical role in controlling gene expression and cellular functions. Its involvement in the Mediator complex makes it a prime target for understanding the mechanisms underlying gene regulation and transcriptional control. Research into MED16 is vital for unraveling complex cellular processes and developing new insights into gene expression regulation in health and disease contexts.
Antibody Name:
MED16 Antibody (PACO17236)
Antibody SKU:
PACO17236
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:5000, IHC:1:25-1:100
Species Reactivity:
Human, Mouse
Immunogen:
Fusion protein of human MED16
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using PACO17236(MED16 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using PACO17236(MED16 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200).
Background:
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Synonyms:
mediator complex subunit 16
UniProt Protein Function:
MED16: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Belongs to the Mediator complex subunit 16 family. 5 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Receptor, misc.Chromosomal Location of Human Ortholog: 19p13.3Cellular Component: membrane; nucleoplasm; Srb-mediator complexMolecular Function: protein binding; receptor activity; thyroid hormone receptor binding; thyroid hormone receptor coactivator activity; transcription coactivator activity; transcription cofactor activityBiological Process: androgen receptor signaling pathway; positive regulation of transcription, DNA-dependent; steroid hormone receptor signaling pathway; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter
